Prenatal Exposure to BPA at ‘Safe’ Levels Can Affect Gene Expression in Developing Rat Brain

New research from North Carolina State University reveals that prenatal exposure to bisphenol A (BPA) at levels below those currently considered safe for humans affects gene expression related to sexual differentiation and neurodevelopment in the developing rat brain.

BPA is a chemical used in a variety of consumer and household products including some food containers. Experimental data has also suggested a link between the chemical and mood or anxiety-related behaviors in children. Currently, the U.S. Food and Drug Administration (FDA) No Observed Adverse Effect Level (NOAEL) for BPA is 50 micrograms per kilogram of body weight per day.

Heather Patisaul, professor of biology at NC State, with Ph.D. candidate Sheryl Arambula, conducted a study exposing gestating rats to levels of BPA both above and below those currently considered to have no adverse effect – including levels as low as 2.5 and 25 micrograms per kilogram of body weight per day – and looked at effects in the brains of their newborn pups.

Arambula and Patisaul found that prenatal BPA exposure, even at the lowest levels, changed the expression of numerous hormone receptors including those for androgen, estrogen, oxytocin and vasopressin in the newborns’ amygdala, a brain structure involved in a wide range of stress and emotional behaviors. Oxytocin, for example, is important for affiliation and pair-bonding, while vasopressin is involved in stress responses. The changes varied depending upon the sex of the newborn and the amount of exposure. Significantly, disruption of genes critical for synaptic transmission and neurodevelopment were also found to be altered, with females appearing to be more sensitive than males.

“Uniquely, we found that low level prenatal BPA exposure can change androgen receptor expression levels in the amygdala,” says Arambula. “In humans, this gene is important for forming differences between male and female brains, which suggests this could be a way by which BPA exposure might alter sex differences in the human brain.”

Patisaul is among a consortium of researchers involved in a multi-year, multi-disciplinary project called CLARITY-BPA, a research initiative that includes the FDA, the National Toxicology Program, the National Institute of Environmental Health Sciences (NIEHS), and 13 academic labs. CLARITY-BPA seeks to understand how BPA affects multiple organ systems. Patisaul’s focus is on brain and behavior. All rats in the study were housed at the National Center for Toxicological Research and followed FDA protocols for exposure. CLARITY-BPA experiments were specifically conceived and conducted to provide the FDA with data it could use to make decisions about human health risks.

“In our previous work, including work for this consortium, we found similar changes in other brain regions including the hypothalamus and hippocampus.” says Patisaul. “There is now a wealth of data showing that BPA can alter neurodevelopment. There is no question that prenatal BPA exposure at levels currently considered safe for humans affects hormone-sensitive gene expression in the developing rodent brain, suggesting that what we consider ‘safe’ for human brains may need to be re-evaluated.”

The researchers’ findings appear in NeuroToxicology. Arambula is first author and Patisaul is corresponding author of the work. Dereje Jima, a specialist with NC State’s Center for Human Health and the Environment, did the bioinformatics analysis. The research was funded by the NIEHS (grants P30ES025128 and U011ES020929).

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Note to editors: An abstract of the paper follows.

“Prenatal bisphenol A (BPA) exposure alters the transcriptome of the neonate rat amygdala in a sex-specific manner: a CLARITY-BPA consortium study”

DOI: 10.1016/j.neuro.2017.10.005

Authors: Sheryl Arambula, Dereje Jima, Heather Patisaul, North Carolina State University
Published: Online in NeuroToxicology

Abstract:
Bisphenol A (BPA) is a widely recognized endocrine disruptor prevalent in many household items. Because experimental and epidemiological data suggest links between prenatal BPA exposure and altered affective behaviors in children, even at levels below the current US FDA No Observed Adverse Effect Level (NOAEL) of 5 mg/kg body weight (bw)/day, there is concern that early life exposure may alter neurodevelopment. The current study was conducted as part of the CLARITY-BPA (Consortium Linking Academic and Regulatory Insights on BPA Toxicity) program and examined the full amygdalar transcriptome on postnatal day (PND) 1, with the hypothesis that prenatal BPA exposure would alter the expression of genes and pathways fundamental to sex specific affective behaviors. NCTR Sprague-Dawley dams were gavaged from gestational day 6 until parturition with BPA (2.5, 25, 250, 2500, or 25000 μg/kg bw /day), a reference estrogen (0.05 or 0.5 μg ethinyl estradiol (EE2)/kg bw/day), or vehicle. PND 1 amygdalae were microdissected and gene expression was assessed with qRT-PCR (all exposure groups) and RNAseq (vehicle, 25 and 250 μg BPA, and 0.5 μg EE2 groups only). Our results demonstrate that that prenatal BPA exposure can disrupt the transcriptome of the neonate amygdala, at doses below the FDA NOAEL, in a sex-specific manner and indicate that the female amygdala may be more sensitive to BPA exposure during fetal development. We also provide additional evidence that developmental BPA exposure can interfere with estrogen, oxytocin, and vasopressin signaling pathways in the developing brain and alter signaling pathways critical for synaptic organization and transmission.

This post was originally published in NC State News.